Increased Cellulose Production from Sucrose with Reduced Levan Accumulation by an<i>Acetobacter</i>Strain Harboring a Recombinant Plasmid
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- TONOUCHI Naoto
- Bio-Polymer Research Co., Ltd.
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- YANASE Hideshi
- Department of Biotechnology, Faculty of Engineering, Tottori University
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- KOJIMA Yukiko
- Bio-Polymer Research Co., Ltd.
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- TSUCHIDA Takayasu
- Bio-Polymer Research Co., Ltd.
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- YOSHINAGA Fumihiro
- Bio-Polymer Research Co., Ltd.
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- HORINOUCHI Sueharu
- Department of Biotechnology, The University of Tokyo
書誌事項
- タイトル別名
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- Increased Cellulose Production from Sucrose with Reduced Levan Accumulation by an Acetobacter Strain Harboring a Recombinant Plasmid.
- Increased Cellulose Production from Suc
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抄録
Cellulose production from sucrose by Acetobacter strains is accompanied by the accumulation of a water-soluble polysaccharide, called levan. To improve cellulose productivity, a levansucrase-deficient mutant, LD-2, was derived from Acetobacter strain 757 and used as a host for the construction of recombinant strains. An LD-2 mutant harboring a plasmid containing the sucrase gene, sucZE3, from Zymomonas mobilis together with zliS, a gene that encodes a secretion-activating factor under the control of the Escherichia coli lac promoter, had sucrase activity and produced much cellulose and little levan in a medium containing sucrose. In addition, a mutant levansucrase gene, mutant sacB, from Bacillus subtilis, which encodes a protein with little levan-forming activity, was generated by site-directed mutagenesis and introduced into the LD-2 mutant. This introduction also resulted in the higher cellulose productivity and little levan.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (5), 833-836, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681451111296
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- NII論文ID
- 110002691901
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXktV2it7c%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL書誌ID
- 4514257
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- PubMed
- 9648211
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可