A Gene Encoding Phosphatidylethanolamine N-Methyltransferase from Acetobacter aceti and Some Properties of its Disruptant.
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- HANADA Takashi
- Department of Fermentation Science, Tokyo University of Agriculture Present address: Research Center, JCR Pharmaceuticals Co. Ltd.
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- KASHIMA Yasuhiro
- Department of Fermentation Science, Tokyo University of Agriculture
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- KOSUGI Akihiko
- Department of Fermentation Science, Tokyo University of Agriculture
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- KOIZUMI Yukimichi
- Department of Fermentation Science, Tokyo University of Agriculture
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- YANAGIDA Fujiharu
- Department of Fermentation Science, Tokyo University of Agriculture
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- UDAKA Shigezo
- Department of Fermentation Science, Tokyo University of Agriculture
書誌事項
- タイトル別名
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- Gene Encoding Phosphatidylethanolamine N Methyltransferase from Acetobacter aceti and Some Properties of its Disruptant
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抄録
Phosphatidylcholine (PC) is a major component of membranes not only in eukaryotes, but also in several bacteria, including Acetobacter. To identify the PC biosynthetic pathway and its role in Acetobacter sp., we have studied Acetobacter aceti IFO3283, which is characterized by high ethanol oxidizing ability and high resistance to acetic acid. The pmt gene of A. aceti, encoding phosphatidylethanolamine N-methyltransferase (Pmt), which catalyzes methylation of phosphatidylethanolamine (PE) to PC, has been cloned and sequenced. One recombinant plasmid that complemented the PC biosynthesis was isolated from a gene library of the genomic DNA of A. aceti. The pmt gene encodes a polypeptide with molecular mass of either 25125, 26216, or 29052 for an about 27-kDa protein. The sequence of this gene showed significant similarity (44.3% identity in the similar sequence region) with the Rhodobacter sphaeroides pmtA gene which is involved in PE N-methylation. When the pmt gene was expressed in E. coli, which lacks PC, the Pmt activity and PC formation were clearly demonstrated. A. aceti strain harboring an interrupted pmt allele, pmt::Km, was constructed. The pmt disruption was confirmed by loss of Pmt and PC, and by Southern blot analyses. The null pmt mutant contained no PC, but tenfold more PE and twofold more phosphatidylglycerol (PG). The pmt disruptant did not show any dramatic effects on growth in basal medium supplemented with ethanol, but the disruption caused slow growth in basal medium supplemented with acetate. These results suggest that the lack of PC in the A. aceti membrane may be compensated by the increases of PE and PG by an unknown mechanism, and PC in A. aceti membrane is related to its acetic acid tolerance.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (12), 2741-2748, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206472855936
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- NII論文ID
- 110002693350
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD38XjtVynsA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 6027377
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- PubMed
- 11826972
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可