Cloned Cytosine Deaminase Gene Expression of Bifidobacterium longum and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors

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  • NAKAMURA Toshiyuki
    <i>Department of Surgery, Shinshu University School of Medicine</i>
  • SASAKI Takayuki
    <i>Department of Surgery, Shinshu University School of Medicine</i>
  • FUJIMORI Minoru
    <i>Department of Surgery, Shinshu University School of Medicine</i>
  • YAZAWA Kazuyuki
    <i>Department of Surgery, Shinshu University School of Medicine</i>
  • KANO Yasunobu
    <i>Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University</i>
  • AMANO Jun
    <i>Department of Surgery, Shinshu University School of Medicine</i>
  • TANIGUCHI Shun'ichiro
    <i>Department of Molecular Oncology and Angiology, Angio-Aging Research Division, Research Center on Aging and Adaptation, Shinshu University School of Medicine</i>

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  • Cloned Cytosine Deaminase Gene Expression of <i>Bifidobacterium longum</i> and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors

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  Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.<br>

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