Identification of a Novel Anti-ice-nucleating Polysaccharide from<i>Bacillus thuringiensis</i>YY529

  • YAMASHITA Yasuhiro
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • KAWAHARA Hidehisa
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • OBATA Hitoshi
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>

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  • Identification of a Novel Anti-ice-nucleating Polysaccharide from Bacillus thuringiensis YY529.

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  Strain YY529, capable of producing some anti-ice-nucleating materials (ANM), was isolated from the surface of a camphor leaf. Strain YY529 was identified as Bacillus thuringiensis from its characteristics and taxonomy; the optimum temperature and pH for producing these ANMs were 30°C and 7.0, respectively. One of the ANM with the highest activities among them was purified from the culture. The molecular weight of the ANM was approximately 130 kDa based on a gel filtration analysis. We confirmed that this ANM was a polysaccharide based on the results of the treatment with a mannosidase and the molish reaction. In addition, the LCMS analysis showed that this anti-ice-nucleating polysaccharide (ANPS) had the polyacetyl-D-glucosamine moiety in its structure. Furthermore, this ANPS showed its ability as a non-freeze agent in a preservative solution for the cryopreservation of cock liver. This is the first report of ANPS as a novel ANM from Bacillus thuringiensis YY529.<br>

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