Identification of a Novel Anti-ice-nucleating Polysaccharide from bacillus thuringiensis YY529

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Abstract

  Strain YY529, capable of producing some anti-ice-nucleating materials (ANM), was isolated from the surface of a camphor leaf. Strain YY529 was identified as <i>Bacillus thuringiensis</i> from its characteristics and taxonomy; the optimum temperature and pH for producing these ANMs were 30°C and 7.0, respectively. One of the ANM with the highest activities among them was purified from the culture. The molecular weight of the ANM was approximately 130 kDa based on a gel filtration analysis. We confirmed that this ANM was a polysaccharide based on the results of the treatment with a mannosidase and the molish reaction. In addition, the LCMS analysis showed that this anti-ice-nucleating polysaccharide (ANPS) had the polyacetyl-<small>D</small>-glucosamine moiety in its structure. Furthermore, this ANPS showed its ability as a non-freeze agent in a preservative solution for the cryopreservation of cock liver. This is the first report of ANPS as a novel ANM from <i>Bacillus thuringiensis</i> YY529.<br>

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 66(5), 948-954, 2002-05-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  22

Cited by:  3

Codes

  • NII Article ID (NAID)
    110002693763
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168451
  • NDL Article ID
    6179904
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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