S-RNases from Self-incompatible and -compatible Apple Cultivars: Purification, Cloning, Enzymic Properties, and Pollen Tube Growth Inhibitory Activity.

• KATOH Naoki

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i> Present address: <i>Aomori Flower Center 21</i>

• GOTO Kazunori

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• ASANO Junpei

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• FUKUSHIMA Kiyoe

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• YAMADA Kenji

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• KASAI Aya

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• LI Tian Zhong

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• TAKANOHA Makoto

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• MIYAIRI Kazuo

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

• OKUNO Toshikatsu

  <i>Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University</i>

Bibliographic Information

Other Title

• S-RNases from Self-incompatible and -compatible Apple Cultivals: Purification, Cloning, Enzymic PRoperties, and Pollen Tube Growth Inhibitory Activity

Abstract

  Four S-RNases (RNase associated with self-incompatibility) were purified from the styles of two apple cultivars (Malus domestica), a self-incompatible cv., Starking Delicious (SD), and a self-compatible cv., Megumi (MG). Each cultivar produced two S-RNases and their enzymatic properties such as specific activity, pH optimum, thermal stability, and molecular mass, were characterized. The four S-RNases inhibited the tube growth of apple pollen in an in vitro bioassay at 25 μg/ml (1.0 μM), but did not distinguish self from non-self pollen. The cDNAs of four S-RNases were cloned, and the nucleotide and deduced amino acid sequences were analyzed. The nucleotide sequence of SD-Se RNase was a new one and the other was identical to that of Sc-RNase of cv. Fuji. In MG one was identical to the sequence of SD-Sc RNase and the other to that of Sa-RNase of cv. Golden Delicious except for one base. From results of the isolation amounts and the Western blot analysis for stylar crude extracts the amount of S-RNases in MG was apparently less than that in SD.<br>

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 66 (6), 1185-1195, 2002

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• S-RNase
• apple
• self-incompatibility
• pollen growth inhibition

Details

• CRID: 1390282681450876416
• NII Article ID: 110002693805
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.66.1185
• COI: 1:CAS:528:DC%2BD38Xlt1Smtb4%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6211020
• PubMed: 12162537
• Web Site: http://id.ndl.go.jp/bib/6211020
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6211020
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.66.1185
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed