Two Low-temperature-inducible Chlorella Genes for ?12 and ω-3 Fatty Acid Desaturase(FAD): Isolation of ?12 and ω-3 fad cDNA Clones, Expression of ?12 fad in Saccharomyces cerevisiae, and Expression of ω-3 fad in Nicotiana tabacum

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  • SUGA Koushirou
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i> <i>Chlorella Industry Co. Ltd.</i>
  • HONJOH Ken-ichi
    <i>Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • FURUYA Naoki
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i>
  • SHIMIZU Hideyuki
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i>
  • NISHI Koutarou
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i>
  • SHINOHARA Fuminori
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i>
  • HIRABARU Yoshie
    <i>Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University</i>
  • MARUYAMA Isao
    <i>Chlorella Industry Co. Ltd.</i>
  • MIYAMOTO Takahisa
    <i>Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>
  • HATANO Shoji
    <i>Graduate School of Health and Social Welfare Science, Nishikyushu University</i>
  • IIO Masayoshi
    <i>Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University</i>

書誌事項

タイトル別名
  • Two Low-temperature-inducible Chlorella Genes for .DELTA.12 and .OMEGA.-3 Fatty Acid Desaturase (FAD): Isolation of .DELTA.12 and .OMEGA.-3 fad cDNA Clones, Expression of .DELTA.12 fad in Saccharomyces cerevisiae, and Expression of .OMEGA.-3 fad in Nicotiana tabacum.
  • Two Low temperature inducible Chlorella Genes for デルタ12 and オメガ 3 Fatty Acid Desaturase FAD Isolation of デルタ12 and オメガ 3 fad cDNA Clones Expression of デルタ12 fad in Saccharomyces cerevisiae and Expression of オメガ 3 fad in Nicotiana tabacum
  • Two Low-temperature-inducible<i>Chlorella</i>Genes for<i>Δ</i>12 and<i>ω</i>-3 Fatty Acid Desaturase (FAD): Isolation of<i>Δ</i>12 and ω-3 fad cDNA Clones,…

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  In an attempt to clarify the involvement of fatty acid desaturases (FADs) in the freezing tolerance of Chlorella vulgaris IAM C-27, developed by hardening, we have isolated cDNA clones for two types of FADs from the Chlorella strain, based on the sequence information of genes for Δ12 and ω-3 FADs, respectively desaturating oleic acid (18:1) to linoleic acid (18:2) and linoleic acid (18:2) to linolenic acid (18:3). The deduced amino acid sequence of the first clone, designated CvFad2, showed about 66% similarity to the microsomal Δ12 FADs from several higher plants and this gene had Δ12 FAD activity when expressed in Saccharomyces cerevisiae. The predicted protein encoded by a second gene, designated CvFad3, showed about 60% similarity to the microsomal and plastidial ω-3 FADs from several higher plants. The features of the amino acid sequences of the C- and N-terminal regions of CvFAD3 and fatty acid analysis of polar lipids in transgenic tobacco plant expressing the CvFad3 gene suggested that this gene encodes the microsomal ω-3 FAD. Southern blot analysis showed that both genes were single-copy genes in the genome of the Chlorella strain. Different transcriptional patterns were observed with the two genes during hardening in Northern blot analysis.<br>

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