<i>In vivo</i>Visualization of the Distribution of a Secretory Protein in<i>Aspergillus oryzae</i>Hyphae Using the RntA-EGFP Fusion Protein

• MASAI Kumiko

  <i>Department of Biotechnology, The University of Tokyo</i>

• MARUYAMA Jun-ichi

  <i>Department of Biotechnology, The University of Tokyo</i> <i>Bio-oriented Technology Research Advancement Institution (BRAIN)</i>

• NAKAJIMA Harushi

  <i>Department of Biotechnology, The University of Tokyo</i>

• KITAMOTO Katsuhiko

  <i>Department of Biotechnology, The University of Tokyo</i>

書誌事項

タイトル別名

• In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein.

抄録

  A fusion gene encoding ribonuclease T₁-EGFP (rntA-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in Aspergillus oryzae. The successful secretion of the intact RntA-EGFP fusion protein was detected by fluorescence measurement and Western analysis. With use of the RntA-EGFP system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. Cold or heat shock during growth caused the accumulation of EGFP fluorescence in vacuoles.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 67 (2), 455-459, 2003

  公益社団法人 日本農芸化学会

キーワード

• <i>Aspergillus oryzae</i>
• secretory pathway
• fusion protein
• EGFP

詳細情報

• CRID: 1390282681450390912
• NII論文ID: 110002694085
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.67.455
• COI: 1:CAS:528:DC%2BD3sXhvFemt7Y%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6460762
• PubMed: 12729022
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6460762
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.455
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可