Rapid Measurement of Phytate in Raw Soymilk by Mid-infrared Spectroscopy
-
- ISHIGURO Takahiro
- <i>Graduate School of Agriculture, Iwate University</i>
-
- ONO Tomotada
- <i>Graduate School of Agriculture, Iwate University</i>
-
- NAKASATO Katsuhiko
- <i>Graduate School of Agriculture, Iwate University</i>
-
- TSUKAMOTO Chigen
- <i>Graduate School of Agriculture, Iwate University</i>
-
- SHIMADA Shinji
- <i>National Agricultural Research Center for Tohoku Region</i>
この論文をさがす
抄録
The phytate content in soymilk is known to affect tofu curdling. A rapid measurement of phytate from a water extract of soybean (raw soymilk) in an early stage of tofu processing was investigated using mid-infrared spectroscopy (IR) with an ATR accessory. IR absorption of phytate was observed from 1200 cm-1 to 900 cm-1, and saccharide and protein in the extract also had IR absorption in the same region. In order to separate phytate from other components, the phytate was precipitated completely by the addition of calcium under alkaline condition (pH 11.5). The precipitate was dissolved in citrate buffer (pH 6.0) and then used for IR measurement. The absorbance at 1070 cm-1 correlated well with the phytate content of the soymilk. The measurement of phytate in raw soymilk can be done rapidly by FT-IR measurement with an ATR accessory and gives reproducible values, which can be used for the measurement of phytate content in various soybeans for tofu making.<br>
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 67 (4), 752-757, 2003
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681451845504
-
- NII論文ID
- 110002694130
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD3sXjslOmtLw%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 6516924
-
- PubMed
- 12784614
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可