Pollen Dispersal in a Hinoki (Chamaecyparis obtusa) Seed Orchard Detected Using a Chloroplast DNA Marker

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Abstract

Pollen dispersal was estimated in two test plots in a hinohi (Chamaecyparis obtusa) seed orchard using a chloroplast DNA marker, the spacer region between the trnD and trnY genes, and SSCP (single strand conformation polymorphism). In Plot 1. 2,020 seeds from 40 trees within 30 m of the marker tree were analyzed using the PCR-SSCP method. In Plot 2, 1,850 seeds from 37 trees were analyzed in the same manner. The results revealed that the maximum pollen dispersal distance in the two plots exceeded 25 m. Pollen dispersal appeared to be inversely proportional to the distance from the marker tree. The effective pollen dispersal was suggested to be less than about 20 m in a mature hinoki seed orchard. Adjacent trees had an excessive influence when the pollen density was increased by artificial flower stimulation. Therefore, it was suggested that seed production better resembles ideal random mating when carried out as naturally as possible. In conclusion, the SSCP chloroplast DNA marker was a useful tool for amassing basic information on pollen management in seed orchards of coniferous species.

Journal

  • Journal of forest research

    Journal of forest research 6(1), 7-12, 2001-02-16

    Japanese Forestry Society

References:  33

Codes

  • NII Article ID (NAID)
    110002694581
  • NII NACSIS-CAT ID (NCID)
    AA11074682
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13416979
  • Data Source
    CJP  NII-ELS  NDL-Digital 
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