Intracellular Delivery of Proteins into Mammalian Living Cells by Polyethylenimine-Cationization
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- Futami Junichiro FUTAMI JUNICHIRO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- Kitazoe Midori KITAZOE MIDORI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- Maeda Takashi [他] MAEDA TAKASHI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- NUKUI EMIKO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SAKAGUCHI MASAKIYO
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- KOSAKA JUN
- Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
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- MIYAZAKI MASAHIRO
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- KOSAKA MEGUMI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- TADA HIROKO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SENO MASAHARU
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SASAKI JUNZO
- Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
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- HUH NAM-HU
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- NAMBA MASAYOSHI
- Niimi College
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- YAMADA HIDENORI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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Author(s)
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- Futami Junichiro FUTAMI JUNICHIRO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- Kitazoe Midori KITAZOE MIDORI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- Maeda Takashi [他] MAEDA TAKASHI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- NUKUI EMIKO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SAKAGUCHI MASAKIYO
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- KOSAKA JUN
- Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
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- MIYAZAKI MASAHIRO
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- KOSAKA MEGUMI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- TADA HIROKO
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SENO MASAHARU
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
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- SASAKI JUNZO
- Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
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- HUH NAM-HU
- Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
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- NAMBA MASAYOSHI
- Niimi College
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- YAMADA HIDENORI
- Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
Abstract
In the post-genomic era, there is pressing need for development of protein manipulation methodology to analyze functions of proteins in living cells. For this purpose, techniques to deliver functional proteins into living cells are currently being evaluated as alternative approaches to the introduction of transcriptionally active DNA. Here, we describe a novel method for efficient protein transduction into living cells in which a protein is simply cationized with polyethylenimine (PEI) by limited chemical conjugation. PEI-cationized proteins appear to adhere to the cell surface by ionic charge interaction and then internalize into cells in a receptor-and transporter-independent fashion. Since PEI is an organic macromolecule with a high cationic-charge density, limited coupling with PEI results in endowment of sufficient cationic charge to proteins without causing serious decline in their fundamental functions. A number of PEI-cationized proteins, such as ribonuclease (RNase), green fluorescent protein (GFP) and immunoglobulin (IgG), efficiently entered cells and functioned in the cytosol. Our results suggest that protein cationization techniques using PEI will be useful for the development of protein transduction technology.
Journal
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- Journal of bioscience and bioengineering
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Journal of bioscience and bioengineering 99(2), 95-103, 2005-02-25
The Society for Biotechnology, Japan
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