Intracellular Delivery of Proteins into Mammalian Living Cells by Polyethylenimine-Cationization

Author(s)

    • Kitazoe Midori KITAZOE MIDORI
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
    • NUKUI EMIKO
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
    • SAKAGUCHI MASAKIYO
    • Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
    • KOSAKA JUN
    • Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
    • MIYAZAKI MASAHIRO
    • Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
    • KOSAKA MEGUMI
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
    • TADA HIROKO
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
    • SENO MASAHARU
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University
    • SASAKI JUNZO
    • Department of Cytology and Histology, Okayama University Graduate School of Medicine and Dentistry
    • HUH NAM-HU
    • Department of Cell Biology, Okayama University Graduate School of Medicine and Dentistry
    • YAMADA HIDENORI
    • Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University

Abstract

In the post-genomic era, there is pressing need for development of protein manipulation methodology to analyze functions of proteins in living cells. For this purpose, techniques to deliver functional proteins into living cells are currently being evaluated as alternative approaches to the introduction of transcriptionally active DNA. Here, we describe a novel method for efficient protein transduction into living cells in which a protein is simply cationized with polyethylenimine (PEI) by limited chemical conjugation. PEI-cationized proteins appear to adhere to the cell surface by ionic charge interaction and then internalize into cells in a receptor-and transporter-independent fashion. Since PEI is an organic macromolecule with a high cationic-charge density, limited coupling with PEI results in endowment of sufficient cationic charge to proteins without causing serious decline in their fundamental functions. A number of PEI-cationized proteins, such as ribonuclease (RNase), green fluorescent protein (GFP) and immunoglobulin (IgG), efficiently entered cells and functioned in the cytosol. Our results suggest that protein cationization techniques using PEI will be useful for the development of protein transduction technology.

Journal

  • Journal of bioscience and bioengineering

    Journal of bioscience and bioengineering 99(2), 95-103, 2005-02-25

    The Society for Biotechnology, Japan

References:  44

Cited by:  3

Codes

  • NII Article ID (NAID)
    110002695633
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13891723
  • NDL Article ID
    7267099
  • NDL Source Classification
    ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
  • NDL Call No.
    Z53-S65
  • Data Source
    CJP  CJPref  NDL  NII-ELS 
Page Top