3-Dehydroquinate Production by Oxidative Fermentation and Further Conversion of 3-Dehydroquinate to the Intermediates in the Shikimate Pathway

DOI Web Site Web Site PubMed 被引用文献10件 参考文献39件
  • ADACHI Osao
    <i>Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University</i>
  • TANASUPAWAT Somboon
    <i>Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University</i>
  • YOSHIHARA Nozomi
    <i>Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University</i>
  • TOYAMA Hirohide
    <i>Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University</i>
  • MATSUSHITA Kazunobu
    <i>Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University</i>

この論文をさがす

抄録

  3-Dehydroquinate production from quinate by oxidative fermentation with Gluconobacter strains of acetic acid bacteria was analyzed for the first time. In the bacterial membrane, quinate dehydrogenase, a typical quinoprotein containing pyrroloquinoline quinone (PQQ) as the coenzyme, functions as the primary enzyme in quinate oxidation. Quinate was oxidized to 3-dehydroquinate with the final yield of almost 100% in earlier growth phase. Resting cells, dried cells, and immobilized cells or an immobilized membrane fraction of Gluconobacter strains were found to be useful biocatalysts for quinate oxidation. 3-Dehydroquinate was further converted to 3-dehydroshikimate with a reasonable yield by growing cells and also immobilized cells. Strong enzyme activities of 3-dehydroquinate dehydratase and NADP-dependent shikimate dehydrogenase were detected in the soluble fraction of the same organism and partially fractionated from each other. Since the shikimate pathway is remote from glucose in the metabolic pathway, the entrance into the shikimate pathway from quinate to 3-dehydroquinate looks advantageous to produce metabolic intermediates in the shikimate pathway.<br>

収録刊行物

被引用文献 (10)*注記

もっと見る

参考文献 (39)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ