Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits Its Na+ Transport

• KATO Yasuhiro

  <i>Graduate School of Bioagricultural Sciences, Nagoya University</i>

• HAZAMA Akihiro

  <i>Fukushima Medical University</i>

• YAMAGAMI Mutsumi

  <i>Institute of Environmental Sciences</i>

• UOZUMI Nobuyuki

  <i>Graduate School of Bioagricultural Sciences, Nagoya University</i> <i>Bioscience and Biotechnology Center, Nagoya University</i>

Bibliographic Information

Other Title

• Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits its Na〔+〕 Transport
• Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits Its Na⁺Transport

Abstract

  Eight transmembrane segments in the Arabidopsis Na⁺ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na⁺ transport in Xenopus ooytes as detected by electrophysiological measurements.<br>

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 67 (10), 2291-2293, 2003

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• AtHKT1
• transporter
• sodium
• membrane protein
• <i>Xenopus laevis</i> oocyte

Details

• CRID: 1390282681452455168
• NII Article ID: 110002698778
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.67.2291
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6731296
• PubMed: 14586126
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6731296
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.2291
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed