アブラナ科野菜根こぶ病菌の単一休眠胞子による簡便な接種法

• 景山 幸二

  岐阜大学農学部

• 上村 順子

  岐阜大学農学部 農業環境技術研究所

• 百町 満朗

  岐阜大学農学部

書誌事項

タイトル別名

• A Simple Inoculation Method with a Single Resting Spore of Plasmodiophora brassicae.
• アブラナ科野菜根こぶ病菌の単一休眠胞子による簡便な接種法〔英文〕
• アブラナカ ヤサイ ネコブビョウキン ノ タンイツ キュウミン ホウシ ニヨル

抄録

Single inoculation method using a single resting spore of Plasmodiophora brassicae was developed. Single resting spore was obtained by placing a 0.5μl drop of spore suspension, which was adjusted to the concentration of 2000 spores per ml, on a piece of sterilized cover glass with a micropipette. The drop was checked under an inverted microscope to ensure that it exactly carried one spore alone. The cover glass carrying a single spore was placed on the surface of autoclaved soil in a pot. One- or two-day-old seedling of Chinese cabbage was inoculated by placing over the spore drop, covered with a small amount of soil, and grown in a growth chamber. The inoculation method could give an efficient infection. Pathogenicity of the single-spore isolates obtained was compared with their original populations to the host lines of European Clubroot Differential set, and was not always identical in those combinations.

収録刊行物

• 日本植物病理学会報

  日本植物病理学会報 61 (5), 415-418, 1995

  日本植物病理学会

キーワード

• crucifers
• <i>Plasmodiophora brassicae</i>
• single-resting-spore inoculation

詳細情報

• CRID: 1390001206403441920
• NII論文ID: 110002732331
• NII書誌ID: AN0019269X
• DOI: 10.3186/jjphytopath.61.415
• ISSN: 18820484
• ISSN: 00319473
• NDL書誌ID: 3643375
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030532109
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3643375
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可