Responses of Callus Tissues of Rice, to Inoculation of Conidia of <i>Pyricularia oryzae</i> Cavara and other Fungi

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  • OGASAWARA Nagahiro
    Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
  • UCHIYAMA Takeo
    Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
  • OOHASHI Tetsuo
    Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
  • TANAKA Hirosato
    Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University

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Other Title
  • いもち病菌およびその他２，３の糸状菌胞子接種に対するイネカルスの反応
  • イモチビョウキン オヨビ ソノタ 2 3 ノ シジョウキン ホウシ セッシュ

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Abstract

Callus cultures were derived from rice seedlings by growing on Murashige-Skoogs medium containing 2.2ppm of 2.4-Dichlorophenoxyacetic acid. The callus of cultivars, Te-Tep and Norin 20 turned brown 24hr to 48hr after inoculation with Pyricuralia oryzae P2, Cochliobolus miyabeanus, Aspergillus oryzae or other pathogenic and non-pathogenic fungi. P. oryzae formed appressoria and infection pegs on rice callus 24-48hr after inoculation. C. miyabeanus, invaded tissues without forming apparent appressorium. A saprophytic fungus, A. oryzae, could also invade the callus tissues. Serial sections of infected callus tissues were prepared. Brown granules or paste* like materials were present on the outermost layer of callus tissues together with spores and mycelia. Degeneration of nuclei or cytoplasm of callus tissues was not observed at the early stage of fungus infection. Increase of peroxidase activity was observed in Te-Tep and Norin 20 after the inoculation with P. oryzae P2. But neither P. oryzae nor A. oryzae caused an increase in the rate of oxygen uptake of Te-Tep and Norin 20. It appears that callus tissues of rice turned susceptible to P. oryzae and to other fungi during consequtive transfers to fresh medium.

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