Differential Staining of Cartilage and Bone in Fetal Mouse Skeleton by Alcian Blue and Alizarin Red S

• INOUYE Minoru

  Institute for Developmental Research, Aichi Prefectural Colony

Abstract

Full-term mouse fetuses were fixed in 95% ethanol, skinned and eviscerated. The specimens were stained with 70% ethanol containing 0.015% alcian blue, 0.005% alizarin red S and 5% acetic acid at 37℃ for 2-3 days. After washing in water, the specimens were put in 1% aqueous KOH until skeletons were visible through surrounding tissue. Then the specimens were cleared through 20% aqueous glycerin containing I% KOH, 50% glycerin and 80% glycerin, and stored in 100% glycerin. Cartilage was stained blue and ossified skeleton was stained red.

Journal

• official journal of Congeital Anomalies Research Association of Japan

  official journal of Congeital Anomalies Research Association of Japan 16 (3), 171-173, 1976-09-30

  The Japanese Teratology Society

Details

• CRID: 1390564238072446592
• NII Article ID: 110002753882
• NII Book ID: AN10066760
• DOI: 10.24540/cgafa.16.3_171
• ISSN: 24331503
• ISSN: 00372285
• Web Site: https://search.jamas.or.jp/link/ui/1978063012
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed