高分子化補酵素の合成とその酵素リアクタ-への応用 Macromolecular derivatives of NAD(P) and their application in model enzyme reactors.

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著者

    • 卜部 格 URABE ITARU
    • 大阪大学工学部発酵工学科 Department of Fermentation Technology, Faculty of Engineering, Osaka University

抄録

1. An NAD derivative carrying a vinyl group was copolymerized with acrylamide or methacrylamide, and various water-soluble macromolecular NAD derivatives (polymeric NAD derivatives) were obtained. I investigated the coenzymic properties of the polymeric NAD derivatives and concluded that smaller NAD derivatives and lower NAD contents in the polymer chain had higher cofactor activity.2. Poly(ethylene glycol)-bound NAD (PEG-NAD) was prepared by coupling N^6-(2-carboxyethyl)-NAD to one termial of α, ω-diaminopoly(etylene glycol) with water-soluble carbodiimide. The kinetic properties of NAD(H), N^6-(2-carboxyethyl)-NAD(H) and PEG-NAD(H) were investigated with various dehydrogenases from different sources.3. NADP derivatives alkylated at the 2'-phosphate and/or 6-amino groups of the adenosyl moiety were prepared, and the effects of these modifications of NADP on its cofactor activity for various dehydrogenases were investigated. Then, poly(ethylene glycol)-bound NADP (PEG-NADP) was prepared by selective alkylation of the 6-amino group of NAD. PEG-NADP has good cofactor activity for some dehydrogenases but not for isocitrate or glucose dehydrogenases.4. The kinetic properties of a continuous enzyme reactor containing rabbit muscle lactate dehydrogenase, horse liver alcohol dehydrogenase, and PEG-NAD were investigated experimentally and theoretically. The steady-state behavior of the enzyme reactor was explained semi-quantitatively by a simple kinetic model. The operational stability of a continuous enzyme reactor containing PEG-NAD and thermostable dehydrogenases was also studied.

収録刊行物

  • 醗酵工学会誌

    醗酵工学会誌 64(2), p77-97, 1986-03

    日本生物工学会

各種コード

  • NII論文ID(NAID)
    110002777892
  • NII書誌ID(NCID)
    AN00205237
  • 本文言語コード
    JPN
  • ISSN
    03856151
  • NDL 記事登録ID
    3081760
  • NDL 刊行物分類
    PA61(醗酵・微生物工学)
  • NDL 雑誌分類
    ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
  • NDL 請求記号
    Z17-395
  • データ提供元
    NDL  NII-ELS  NDL-Digital 
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