紫外部吸収検出器付高速液体クロマトグラフィーによる血清及び肝臓中のユビキノンの定量

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タイトル別名
  • Determination of Ubiquinone in Serum and Liver by High-Speed Liquid Chromatography with Ultraviolet Absorption Detector
  • シガイブ キュウシュウ ケンシュツキツキ コウソク エキタイ クロマトグラフィ

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抄録

A Method for the determination of ubiquinone homologues (UQ) in serum and liver is described. Serum or liver homogenate was saponified with methanolic alkaline solution after addition of 2,3,6-trimethyl-5-nonaprenyl-1,4-benzoquinone (TQ-9) as an internal standard and then extracted with n-hexane. The n-hexane extract was evaporated under N_2 gas at 30℃, and the residue was dissolved in dioxane. The sample solution was applied to the high-speed liquid chromatography (HSLC) using a Permaphase ODS column and ethanol・water (80 : 20) as a mobile phase. The peaks of UQ and TQ-9 were not disturbed by other fat-soluble substances when an on-line high-sensitive multiple wavelength detector set at 275 nm (absorption maximum of UQ) was used as a detector. The proposed HSLC method was found to be more simple and specific than the other conventional determination methods.

収録刊行物

  • ビタミン

    ビタミン 51 (3), 111-117, 1977

    公益社団法人 日本ビタミン学会

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