バイオファクター・セレンを含む新規酵素の発見と肺がん細胞のセレン代謝特性の解明  [in Japanese] Discovery of a New Enzyme That Contains Biofactor Selenium and Characterization of Selenium Metabolism in Human Lung Adenocarcinoma Cells  [in Japanese]

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Author(s)

    • 田村 隆 TAMURA Takashi
    • 岡山大学・大学院自然科学研究科 The Graduate School of Natural Science and Technology Okayama University

Abstract

Selenium is an essential trace element for mammals, birds, and some bacteria. Its remarkable biological effects in eukaryotes may be related to unique functions of various selenoproteins. A^<75>Se-labeled protein was purified from a human lung adenocarcinoma cell line, NCI-H441 . A homodimer of 57-kDa subunits contained selenium in the form of selenocysteine. The selenoprotein contained FAD as a prosthetic group and catalyzes NADPH-dependent reduction of insulin in the presence of thioredoxin. The subunit composition and catalytic properties were identical to those of mammalian thioredoxin reductase (TrxR). This was the first report on the biochemical relevance between selenium and Trx-mediated metabolic regulation. Interestingly, human lung adenocarcinoma cells produced the selenoenzyme TrxR over hundred times that of normal mammalian cells. A Iabile selenium donor compound monoselenophosphate, required for selenoprotein biosynthesis, is synthesized from selenide and ATP by selenophosphate synthetase (SPS).

Journal

  • VITAMINS

    VITAMINS 79(3), 143-153, 2005

    THE VITAMIN SOCIETY OF JAPAN

References:  45

Codes

  • NII Article ID (NAID)
    110002880725
  • NII NACSIS-CAT ID (NCID)
    AN00207833
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    0006-386X
  • NDL Article ID
    7292954
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z19-36
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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