Determination of testosterone in an individual shell of Thais clavigera by ELISA.

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  • LU Ming
    Department of Applied Biological Chemistry, The University of Tokyo
  • HORIGUCHI Toshihiro
    Environmental Chemistry Division, National Institute for Environmental Studies
  • SHIRAISHI Hiroaki
    Environmental Chemistry Division, National Institute for Environmental Studies
  • SHIBATA Yasuyuki
    Environmental Chemistry Division, National Institute for Environmental Studies
  • ABO Mitsuru
    Department of Applied Biological Chemistry, The University of Tokyo
  • OKUBO Akira
    Department of Applied Biological Chemistry, The University of Tokyo
  • YAMAZAKI Sunao
    Faculty of Environmental Studies, Nagasaki University

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Other Title
  • ＥＬＩＳＡ法によるイボニシ中のテストステロンの個体別分析
  • ELISAホウ ニ ヨル イボニシ チュウ ノ テストステロン ノ コタイ ベツ ブンセキ

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Abstract

A determination method for testosterone in the reproductive organ of an individual shell of a marine gastropod, Thais clavigera, was established by ELISA (enzyme-linked immunosorbent assay). Large deviation in analytical values often encountered in ELISA was overcome both by selecting the ELISA kit used and by extensively purifying the fraction to be analyzed in the pre-treatment steps. These resulted in the ELISA values comparable to those by GC/MS. Based on this method, significantly higher testosterone content was confirmed in the female imposex-suffered shells collected from organotin-contaminated seashores than normal ones.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 51 (1), 21-27, 2002

    The Japan Society for Analytical Chemistry

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