Separation and determination of n-alkylamines by reversed-phase high-performance liquid chromatography coupled with merging zone derivatization to water-soluble Schiff bases

• TAKAYANAGI Toshio

  Department of Chemistry, Faculty of Science, Okayama University

• DRIOUICH Rim

  Department of Chemistry, Faculty of Science, Okayama University

• SAWAI Kuniko

  Department of Chemistry, Faculty of Science, Okayama University

• LI Qiong

  Department of Chemistry, Faculty of Science, Okayama University

• OSHIMA Mitsuko

  Department of Chemistry, Faculty of Science, Okayama University

• MOTOMIZU Shoji

  Department of Chemistry, Faculty of Science, Okayama University

Bibliographic Information

Other Title

• 水溶性シッフ塩基へのマージングゾーン誘導体化／逆相分配高速液体クロマトグラフィーによるｎ‐アルキルアミン類の分離定量
• 水溶性シッフ塩基へのマージングゾーン誘導体化/逆相分配高速液体クロマトグラフィーによるn-アルキルアミン類の分離定量
• スイヨウセイ シッフ エンキ エ ノ マージングゾーン ユウドウタイカ ギャクソウ ブンパイ コウソク エキタイ クロマトグラフィー ニ ヨル n アルキルアミンルイ ノ ブンリ テイリョウ

Abstract

Separation and determination of n-alkylamines were investigated with anionic aldehydes to form water-soluble Schiff bases by using reversed-phase high-performance liquid chromatography (RP-HPLC). Two labeling reagents of salicylaldehyde-5-sulfonate (SAS) and 4-(4-sulfophenylazo)-1-hydroxy-2-naphthaldehyde (SPAHNA) were investigated. Sample solution containing the analytes and reagent solution were simultaneously injected and merged in the flow line, and water-soluble Schiff bases were formed in the reaction tube with its length of 5 m and at 70°C. The Schiff bases were resolved with water-methanol eluent on a reversed-phase column and photometrically detected. The entire analysis time including on-line derivatization, column separation and detection was accomplished within 25 min with SAS and 18 min with SPAHNA. The determination range for the n-alkylamines are 0∼50 μM with the limit of detections at 0.5∼1.0 μM for SAS and 0.08∼0.20 μM for SPAHNA.<br>

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 53 (7), 723-728, 2004

  The Japan Society for Analytical Chemistry

Keywords

• <i>n</i>-alkylamines
• salicylaldehyde-5-sulfonate
• 4-(4-sulfophenylazo)-1-hydroxy-2-naphthaldehyde
• merging zone
• reversed-phase HPLC

Details

• CRID: 1390001204356075648
• NII Article ID: 110002905417
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.53.723
• COI: 1:CAS:528:DC%2BD2cXlvVOmu70%3D
• NDL BIB ID: 7034462
• ISSN: 05251931
• Web Site: https://ousar.lib.okayama-u.ac.jp/14132
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7034462
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku/53/7/53_7_723/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10895818
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed