高速液体クロマトグラフィーによる血清中リファンピシンの測定

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書誌事項

タイトル別名
  • 高速液体クロマトグラフィーによる血清中リファンピンの測定
  • Determination of rifampicin in serum by high-performance liquid chromatography
  • コウソク エキタイ クロマトグラフィー ニヨル ケッセイチュウ リファンピシン

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抄録

A simple, rapid and reproducible method for determination of rifampicin in serum by high-performance liquid chromatography has been developed. Only 20 μl of serum is required. Serum proteins are precipitated with 100 μl of methanol and rifampicin is dissolved into the methanol solution. After the centrifugation, 90 μl of the supernatant is injected into the chromatograph equipped with a Model U6K injector, a 3.9 mm × 30 cm μBondapak C18 column, a Model 440 UV detector set at 0.01 AUFS (340 nun) and a 6000 A solvent delivery system (Waters Assoc. Inc., ). The flow rate of the mobile phase of 38 % acetonitrile solution containing 0.01 M sodium acetate (pH 7.0) is 1.5 ml/min.<BR>The retention time for rifampicin is 9 min. The amount of rifampicin is determined by measuring peak heights. The limit of detection is 0.5μg/ml of serum. Within-run and day-to-day reproducibility for 8.0 μg/ml are ± 1.0% (n =10) and ± 1.5% (n =10) respectively. The proposed method can be completed in less than 13 min and had been applied for the serum of volunteers taking rifampicin.

収録刊行物

  • 分析化学

    分析化学 31 (4), 175-179, 1982

    公益社団法人 日本分析化学会

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