Liquid-chromatographic determination of halogenated bisphenols based on intramolecular excimer-forming fluorescence derivatization

• TSUNETOMO Shigekatsu

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YOSHIDA Hideyuki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• TODOROKI Kenichiro

  Faculty of Pharmaceutical Sciences, Fukuoka University

• HAYAMA Tadashi

  Faculty of Pharmaceutical Sciences, Fukuoka University Environmental Science Center, Foundation for Kyushu Environmental and Occupational Health

• NOHTA Hitoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• KUROKI Hiroaki

  Daiichi College of Pharmaceutical Sciences

• YAMAGUCHI Masatoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

Bibliographic Information

Other Title

• エキシマー蛍光誘導体化法によるハロゲン化ビスフェノール類の液体クロマトグラフ分析
• エキシマー ケイコウ ユウドウタイカホウ ニ ヨル ハロゲンカ ビスフェノールルイ ノ エキタイ クロマトグラフ ブンセキ

Abstract

A selective determination method for halogenated bisphenols containing tetrabromobisphenol A has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent, 4-(1-pyrene)butanoyl chloride (PBC), followed by reversed-phase chromatography (LC). Bisphenols, containing two phenolic hydroxyl groups in a molecule, were converted to the corresponding dipyrene-labeled derivatives by a reaction with PBC. These derivatives afforded intramolecular excimer fluorescence (440∼540 nm), which could be clearly discriminated from the normal fluorescence (360∼420 nm) emitted from PBC and monopyrene-labeled derivatives. The PBC derivatives could be separated by reversed-phase LC on an ODS column with isocratic elution using aqueous 99% (v/v) acetonitrile as a mobile phase. The detection limits (signal-to-noise ratio = 3) for halogenated bisphenols were femto-mole levels for a 5 μl injection.<br>

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 53 (12), 1501-1505, 2004

  The Japan Society for Analytical Chemistry

Keywords

• halogenated bisphenol
• tetrabromobisphenol A
• liquid chromatography
• excimer fluorescence
• 4-(1-pyrene)butanoyl chloride
• human serum

Details

• CRID: 1390282679330727168
• NII Article ID: 110002910014
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.53.1501
• COI: 1:CAS:528:DC%2BD2cXhtFWru7bK
• NDL BIB ID: 7180617
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7180617
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku/53/12/53_12_1501/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10895990
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed