Liquid Chromatography of 5-hydroxyindoles Based on Pre-Column Fluorescence Derivatization by Photocatalytic Oxidation

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  • 光触媒酸化反応を利用する５‐ヒドロキシインドール類のプレカラム蛍光誘導体化液体クロマトグラフィー
  • 光触媒酸化反応を利用する5-ヒドロキシインドール類のプレカラム蛍光誘導体化液体クロマトグラフィー
  • ヒカリ ショクバイ サンカ ハンノウ オ リヨウ スル 5 ヒドロキシインドールルイ ノ プレカラム ケイコウ ユウドウタイカ エキタイ クロマトグラフィー

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Abstract

We have developed a fluorescence derivatization method of 5-hydroxyindoles (serotonin, 5-hydroxyindole-3-acetic acid, N -acetylserotonin) based on benzylamine derivatization, followed by photocatalytic oxidation. In the present study, a photocatalyst, which was titanium dioxide supported on a diatomaceous earth particle, was used instead of potassium hexacyanoferrate(III) as a chemical oxidizing agent employed in the conventional method. The resulting fluorescent derivatives were separated by reversed-phase liquid chromatography on an ODS column by using a mixture of acetonitrile and 250 mM acetate buffer (pH 4.6) and detected spectrofluorimetrically at 465 nm with excitation at 350 nm. The detection limit (signal-to-noise ratio = 3) for N -acetylserotonin was 16 fmol per 20 μl injection.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 54 (12), 1211-1214, 2005

    The Japan Society for Analytical Chemistry

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