SEM Observation of Collagen Fibrils Secreted from the Body Surface of Osteoblasts on a CO_3apatite-collagen Sponge

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Author(s)

    • HIRATA Isao
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
    • NOMURA Yuji
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
    • TABATA Hitoshi
    • Center for Nano-technology, The Institute of Scientific and Industrial Research, Osaka University
    • MIAKE Yasuo
    • Department of Ultrastructural Science, Tokyo Dental College
    • OKAZAKI Masayuki
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University

Abstract

The secretion of collagen by osteoblasts was observed by scanning electron microscopy (SEM). Osteoblast-like cells were cultured on a CO<sub>3</sub>apatite-collagen sponge reinforced with a porous HAp frame. After three days' incubation, a number of newly created matrix fibrils, forming a network structure, were observed at the cell surface. SEM also showed that osteoblasts secreted collagen fibrils from their membrane, and that the collagen fibrils were twisted together. When collagen in an aqueous sol solution was sprayed onto the extremely smooth surface of a mica plate to support the secretion of osteoblasts, a collagen network structure could be clearly observed with atomic force microscopy (AFM). With this <i>in vitro</i> phenomenon, we could confirm the formation of collagen network structure without biological function. Therefore, it was suggested that the CO<sub>3</sub>apatite-collagen sponge used in this study is a favorable scaffold biomaterial, on which osteoblasts could produce the unmistakable, characteristic extracellular matrix for mineralization. For therapeutic use of hard tissue biomaterials, collagen formation as an extracellular matrix (ECM) is very important because mineralization is subsequent to ECM.

Journal

  • Dental Materials Journal

    Dental Materials Journal 24(3), 460-464, 2005-09-01

    The Japanese Society for Dental Materials and Devices

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    110002983730
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    02874547
  • Data Source
    CJP  CJPref  NII-ELS  J-STAGE  NDL-Digital 
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