SEM Observation of Collagen Fibrils Secreted from the Body Surface of Osteoblasts on a CO3apatite-collagen Sponge
-
- HIRATA Isao
- Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
-
- NOMURA Yuji
- Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
-
- TABATA Hitoshi
- Center for Nano-technology, The Institute of Scientific and Industrial Research, Osaka University
-
- MIAKE Yasuo
- Department of Ultrastructural Science, Tokyo Dental College
-
- YANAGISAWA Takaaki
- Department of Ultrastructural Science, Tokyo Dental College
-
- OKAZAKI Masayuki
- Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
この論文をさがす
抄録
The secretion of collagen by osteoblasts was observed by scanning electron microscopy (SEM). Osteoblast-like cells were cultured on a CO3apatite-collagen sponge reinforced with a porous HAp frame. After three days' incubation, a number of newly created matrix fibrils, forming a network structure, were observed at the cell surface. SEM also showed that osteoblasts secreted collagen fibrils from their membrane, and that the collagen fibrils were twisted together. When collagen in an aqueous sol solution was sprayed onto the extremely smooth surface of a mica plate to support the secretion of osteoblasts, a collagen network structure could be clearly observed with atomic force microscopy (AFM). With this in vitro phenomenon, we could confirm the formation of collagen network structure without biological function. Therefore, it was suggested that the CO3apatite-collagen sponge used in this study is a favorable scaffold biomaterial, on which osteoblasts could produce the unmistakable, characteristic extracellular matrix for mineralization. For therapeutic use of hard tissue biomaterials, collagen formation as an extracellular matrix (ECM) is very important because mineralization is subsequent to ECM.
収録刊行物
-
- Dental Materials Journal
-
Dental Materials Journal 24 (3), 460-464, 2005
一般社団法人 日本歯科理工学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679692480640
-
- NII論文ID
- 110002983730
-
- NII書誌ID
- AA10443149
-
- COI
- 1:CAS:528:DC%2BD2MXht1CrsbjP
-
- ISSN
- 18811361
- 02874547
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- NDL-Digital
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可