Immunohistochemical Demonstration of Dihydropyrimidine Dehydrogenase in Normal and Cancerous Tissues

• Kamoshida Shingo

  Departments of Pathology, Fujita Health University School of Medicine

• Shiogama Kazuya

  Departments of Pathology, Fujita Health University School of Medicine

• Matsuoka Hiroshi

  Departments of Surgery, Fujita Health University School of Medicine

• Matsuyama Atsuji

  Departments of Pathology, Fujita Health University School of Medicine

• Shimomura Ryoichi

  Departments of Pathology, Fujita Health University School of Medicine

• Inada Ken-ichi

  Departments of Pathology, Fujita Health University School of Medicine

• Maruta Morito

  Departments of Surgery, Fujita Health University School of Medicine

• Tsutsumi Yutaka

  Departments of Pathology, Fujita Health University School of Medicine

抄録

Dihydropyrimidine dehydrogenase (DPD) is a rate-limiting enzyme in the catabolism of 5-fluorouracil (5-FU). Previous studies have suggested that high enzyme activities and mRNA levels of DPD are involved in the resistance of cancers to 5-FU. For immunohistochemically demonstrating the tumor resistance to 5-FU, we determined the most suitable and reproducible condition of antigen retrieval for DPD localization in formalin-fixed, paraffin-embedded sections, and analyzed DPD expression in various types of normal and cancerous tissues. Pressure cooking in ethylenediaminetetraacetic acid, pH 8.0, was the best choice for retrieving the antigenicity of DPD. In normal tissues, DPD immunoreactivity was consistently detected in squamous epithelia, hepatocytes, Clara cells, ductal/glandular epithelia of various organs, myoepithelial cells, and non-epithelial cells such as macrophages, plasma cells, and part of the endothelial cells and fibroblasts. DPD was strongly expressed in adenocarcinomas of the lung, gallbladder and pancreas, hepatocellular carcinomas, and transitional cell carcinomas of the urinary bladder. Squamous cell carcinomas of the pharynx and lung were moderately reactive. Carcinomas of the breast and stomach revealed less consistent and less intense reactivity. Colorectal adenocarcinomas were not immunoreactive. The polyclonal antibody gave a broader and stronger reactivity than the monoclonal antibody KM1915, particularly in the normal epidermis and skin appendage as well as in squamous cell carcinomas and gastric cancers. The present results hopefully contribute to further investigations, in which the immunohistochemical demonstration of DPD is utilized as a tool of tailor-made chemotherapy for selecting of patients with cancer resistant to 5-FU and its derivatives.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 36 (5), 471-479, 2003

  日本組織細胞化学会

キーワード

• dihydropyrimidine dehydrogenase
• 5-fluorouracil
• chemoresistance
• immunohistochemistry
• antigen retrieval

詳細情報

• CRID: 1390001204862563968
• NII論文ID: 110003144304
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.36.471
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/36/5/36_5_471/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10804475
• Web Site: https://search.jamas.or.jp/link/ui/2004243058
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可