奈良県池原貯水池から検出されたフロリダバス Micropterus salmoides floridanus 由来のミトコンドリア DNA Mitochondrial DNA of the Florida Subspecies of Largemouth Bass Micropterus salmoides floridanus Detected in Ikehara Reservoir, Nara Prefecture, Japan

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抄録

オオクチバスはノーザンバスとフロリダバスの2亜種からなり, 現在日本に広く生息しているのは前者であると考えられている。奈良県の池原貯水池には, 1988年にそれまで生息していたノーザンバスに加えてフロリダバスが移殖された記録がある。池原貯水池を含む全国各地の14集団についてmtDNAのPCR-RFLP分析を行った結果, 池原貯水池の水系から他の水系には認められない遺伝子型が多数検出された。既知の遺伝子型との比較により, これらがフロリダバスに由来するものであることが明らかになった。The largemouth bass Micropterus salmoides consists of two subspecies of the northern subspecies M. s. salmoides and the Florida subspecies M. s. floridanus. It has been widely regarded that largemouth bass, introduced and widespread in Japan, is the northern subspecies. In 1988,the Florida subspecies was also introduced into the Ikehara Reservoir (Nara Prefecture), where northern subspecies were already present. To assess the influence of this introduction, the genetic structure of the largemouth bass population in Ikehara Reservoir and thirteen other locations throughout Japan were examined and compared by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mitochondrial DNA (mtDNA). Analysis of the ND1 region of mtDNA using sixteen restriction enzymes revealed 10 haplotypes (1-10) that form two distinct clusters in the UPGMA tree. One cluster consisted of two haplotypes (1 and 2) commonly detected in most populations throughout Japan, and the other cluster consisted of eight haplotypes (3-10) detected only in Ikehara and its connected Reservoirs. The reported rapid assay to identify mtDNA from two subspecies using D-loop region clearly revealed that eight haplotypes (3-10) were from the Florida subspecies.

The largemouth bass Micropterus salmoides consists of two subspecies of the northern subspecies M. s. salmoides and the Florida subspecies M. s. floridanus. It has been widely regarded that largemouth bass, introduced and widespread in Japan, is the northern subspecies. In 1988, the Florida subspecies was also introduced into the Ikehara Reservoir (Nara Prefecture), where northern subspecies were already present. To assess the influence of this introduction, the genetic structure of the largemouth bass population in Ikehara Reservoir and thirteen other locations throughout Japan were examined and compared by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mitochondrial DNA (mtDNA). Analysis of the ND1 region of mtDNA using sixteen restriction enzymes revealed 10 haplotypes (1-10) that form two distinct clusters in the UPGMA tree. One cluster consisted of two haplotypes (1 and 2) commonly detected in most populations throughout Japan, and the other cluster consisted of eight haplotypes (3-10) detected only in Ikehara and its connected Reservoirs. The reported rapid assay to identify mtDNA from two subspecies using D-loop region clearly revealed that eight haplotypes (3-10) were from the Florida subspecies.

収録刊行物

  • 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries

    日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries 66(5), 805-811, 2000-09-15

    The Japanese Society of Fisheries Science

参考文献:  17件中 1-17件 を表示

被引用文献:  6件中 1-6件 を表示

各種コード

  • NII論文ID(NAID)
    110003145102
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    Journal Article
  • ISSN
    00215392
  • NDL 記事登録ID
    5526042
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP書誌  CJP引用  NDL  NII-ELS  IR  J-STAGE 
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