雌性発生アマゴ卵の第二極体放出阻止最適時期に関する細胞学的検証

• 小林 徹

• 上野 紘一

書誌事項

タイトル別名

• Cyiological Inspection on the Optimum Timing for Retention of the Second Polar Body in Early Gynogenetic Eggs of Amago Salmon.
• シセイ ハッセイ アマゴラン ノ ダイ2キョクタイ ホウシュツ ソシ サイテキ ジキ ニ カンスル サイボウガクテキ ケンショウ

抄録

Behavior of spindle and chromosomes in early development of gynogenetic amago salmon eggs was examined for explication of basic cytological mechanisms to induce gynogenetic diploid using genetically inactivated rainbow trout sperm by UV-irradiation and with heat shock treatment (0°C for 20min) for retention of second polar body. The heat shock treatment that started 30 minutes after insemination of amago salmon eggs incubated at 12.0°C was the optimum condition to induce meiotic gynogenetic diploids compared with other conditions of treatment. At 30 minutes, the eggs were in late anaphase of meiosis II. The percentage of eggs that became diploid larvae when treatment started at 5 to 30 minutes after insemination varied little. When treatment started at anaphase, the survival rate was greater than when treatment started at metaphase. It seems that physical damage to the eggs caused by heat is less at anaphase. The second polar body was retained in fewer eggs when heat treatment started later, in the range of 35 to 60 minutes after insemination. Cytokinesis may have prevented such retention more than the increased distance between chromosomes.

収録刊行物

• 日本水産学会誌

  日本水産学会誌 66 (5), 840-845, 2000

  公益社団法人 日本水産学会

キーワード

• アマゴ
• 雌性発生
• 第二極体放出阻止
• 紡錘体
• 細胞質分裂
• 中央体

詳細情報

• CRID: 1390282681388470272
• NII論文ID: 110003145107
• NII書誌ID: AN00193422
• DOI: 10.2331/suisan.66.840
• ISSN: 1349998X
• ISSN: 00215392
• NDL書誌ID: 5526099
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010620356
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5526099
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/11075447
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可