マツカワ Verasper moseri における三倍体および雌性発生二倍体の誘起  [in Japanese] Induction of triploids and gynogenetic diploids in barfin flounder Verasper moseri  [in Japanese]

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Abstract

マツカワ<i>Verasper moseri</i> の育種基盤の確立を目的に, 紫外線による精子の不活化および染色体倍数化のための好適染色体操作条件を検討した。マツカワ精子は, 紫外線照射強度10mJ/cm<sup>2</sup>以上で不活化された。三倍体誘起の最適低温処理条件は−1.5℃, 受精後9分, 持続時間90分間であった。第二極体放出阻止型雌性発生二倍体では低温処理で−1.5℃, 受精後3~9分, 60~90分間, 圧力処理で650Kg/cm<sup>2</sup>, 受精後7分, 持続時間6分間であった。同様に, 圧力処理による第一卵割阻止型雌性発生二倍体誘起では, 受精後150~240分において孵化仔魚が得られた。

The optimum conditions for inducing triploidy and gynogenesis by cold and pressure shock in barfin flounder <i>Verasper moseri</i> were examined. The sperm of barfin flounder was genetically inactivated by the dose of 10 mJ/cm<sup>2</sup> UV irradiation. Triploids were induced by applying cold shock treatment with −1.5°C at 9 min after fertilization for 90 min duration. In this condition, hatching rate relative to the control was 29.2% and triploidy rate was 90.9%. Meiotic gynogenetic diploids were obtained by fertilizing eggs with UV-irradiated sperm and subsequent cold shock (−1.5°C) at 3-9 min after fertilization for 60-90 min duration and pressure shock (650 kg/cm<sup>2</sup>) at 7 min after fertilization for 6 min duration. Mitotic gynogenetic diploids were obtained by pressure shock (650 kg/cm<sup>2</sup>, 6 min) and applied at 150-240 min after fertilization.

Journal

  • NIPPON SUISAN GAKKAISHI

    NIPPON SUISAN GAKKAISHI 70(2), 145-151, 2004-03-15

    The Japanese Society of Fisheries Science

References:  21

Cited by:  2

Codes

  • NII Article ID (NAID)
    110003145680
  • NII NACSIS-CAT ID (NCID)
    AN00193422
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    00215392
  • NDL Article ID
    6905086
  • NDL Source Classification
    ZR26(科学技術--農林水産--水産)
  • NDL Call No.
    Z18-345
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE  JASI 
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