Extinction of Organelles in Differentiating Epidermis.

• Morioka Kiyokazu

  Department of Clinical Genetics, Faculty of Medicine, University of Tokyo

• Takano-Ohmuro Hiromi

  Department of Pharmacology, Faculty of Medicine, University of Tokyo

• Sameshima Masasumi

  Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science

• Ueno Takashi

  Department of Biochemistry, Juntendo University School of Medicine

• Kominami Eiki

  Department of Biochemistry, Juntendo University School of Medicine

• Sakuraba Hitoshi

  Department of Clinical Genetics, Faculty of Medicine, University of Tokyo

• Ihara Setsunosuke

  Departmentof Biological Science, Faculty of Life and Environmental Science, Shimane University

抄録

In the dorsal skin of fetal rats, the epidermis on day 16 of gestation consisted of a single or a few layers of undifferentiated cells, and it developed to display the full strata, i. e the stratum (s.) corneum (horny layer), s. granulosum (granular layer), s. spinosum (spinous layer), and s. germinativum (basal layer) by the day of birth. Perinuclear electron lucent special areas, which were also detectable by conventional staining under light microscope, appeared in the cells of the s. spinosum or the s. granulosum on the day of birth. We call such an area the “peri-nuclear compartment (PNC) ”. The PNC was not partitioned by membranous structures either. Typical PNCs became recognizable on the day of birth. The cell nuclei seemed to be digested inside of the PNC of the cells located in the outermost area of the s. granulosum or sometimes in the boundary between the s. granulosum and the s. corneum. In the initial phase of nuclear extinction, morphology of the nucleus undergoing degeneration resembles that of apoptotic cells. In comparison to the morphological degeneration of the nucleus, DNA fragmentation was suggested to occur earlier. Localization of cathepsin B and cathepsin D in the s. spinosum and s. granulosum as revealed by immunohistochemistry, together with an electron microscopic study on the appearance of autophagosomes in those areas, suggested a contribution of autophagy to the digestion of organelles including mitochondria and endoplasmic reticula in the terminal differ-entiation of epidermal cells.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 32 (6), 465-476, 1999

  日本組織細胞化学会

キーワード

• Keratinocyte
• Lysosome
• Programmed cell death
• Cathepsin
• Rat

詳細情報

• CRID: 1390001204861580160
• NII論文ID: 110003147061
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.32.465
• ISSN: 13475800
• ISSN: 00445991
• ISSN: http://id.crossref.org/issn/00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/32/6/32_6_465/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10803369
• Web Site: https://search.jamas.or.jp/link/ui/2001068546
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可