HISTOCHEMICAL DEMONSTRATION OF INCREASED ACTIVITY OF γ-GLUTAMYL TRANSPEPTIDASE IN RAT LIVER DURING HEPATOCARCINOGENESIS

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Author(s)

    • HARADA MASAOKI
    • Department of Pathology, St. Marianna University School of Medicine
    • OKABE KAZUHIKO
    • Department of Pathology, St. Marianna University School of Medicine|Department of Medicine, St. Marianna University School of Medicine
    • MASUDA HIROTAKE
    • Department of Pathology, St. Marianna University School of Medicine
    • MIYATA KEIKO
    • Department of Pathology, St. Marianna University School of Medicine
    • ENOMOTO MAKOTO
    • Department of Pathology, St. Marianna University School of Medicine

Abstract

Histochemical demonstration of γ-glutamyl transpeptidase (γ-GTP) during chemically induced hepatocarcinogenesis in the rats was studied. The carcinogens employed were N-OH-FAA (N-hydroxy derivative of N-2-fluorenylacetamide), and aflatoxin B<sub>1</sub>.<br>In the control rats, activity of γ-GTP was demonstrated in the bile duct cells and occasionally along the bile canaliculi, but was not in the hepatocytes. Proliferating bile duct cells including so-called oval cells and cholangiocarcinoma cells, and hepatocytes of hyperplastic areas and nodules and hepatocellular carcinoma induced by N-OH-FAA with or without aflatoxin B<sub>1</sub> revealed positive activity of γ-GTP, histochemically. Only focal demonstration of γ-GTP activity in the hepatocyte was observed in association with a mild hyperplastic change in rats treated with aflatoxin B<sub>1</sub> alone.<br>The elevation of γ-GTP activity in serum and liver tissue with appearance of fetal type of its isoenzyme pattern was also demonstrated biochemically and correlated with the histochemical appearance of γ-GTP activity in the liver tissue from the early phase of hepatobiliary carcinogenesis.<br>These results seem to demonstrate carcino-embryonic nature of the induced γ-GTP in the development of cholangiocellular and hepatocellular carcinomas.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 9(2), 168-179, 1976

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Cited by:  1

Codes

  • NII Article ID (NAID)
    110003148180
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0044-5991
  • Data Source
    CJPref  NII-ELS  J-STAGE  NDL-Digital 
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