Cytochemistry of plasma membranes activated by phorbol myristate acetate and calcium ionophore in the alveolar and peritoneal macrophages of guinea pigs.

• MORIGUCHI KEIICHI

  Department of Anatomy, Kanazawa Medical University

• HIRAI KEI-ICHI

  Department of Anatomy, Kanazawa Medical University

抄録

Ultrastructural H₂O₂ producing sites in guinea pig peritoneal (PM) and alveolar (AM) macrophages stimulated with soluble stimuli were studied using a cerium technique. Electron-dense deposits resulting from H₂O₂ and CeCl₃ reactions were observed on the contacted surface of aggregated PM plasma membranes stimulated by formyl-methionyl-leucyl-phenylalanine (FMLP) or phorbol myristate acetate (PMA). The AM stimulated by FMLP or PMA demonstrated no significant cell aggregation or H₂O₂ production. Although calcium ionophore A23187 did not induce any cell aggregation, deposit formation was apparent on the free surface of plasma membranes. These results indicate that cell-cell contact may have an important role in H₂O₂ production induced by FMLP and PMA, and that H₂O₂ production induced by A23187 may not depend on cell aggregation.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 19 (4), 465-472, 1986

  日本組織細胞化学会

キーワード

• Cell Biology
• Histology
• Physiology
• Biochemistry
• Pathology and Forensic Medicine

詳細情報

• CRID: 1390001204861281664
• NII論文ID: 110003150002
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.19.465
• COI: 1:CAS:528:DyaL2sXjtFaqtA%3D%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/19/4/19_4_465/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10801831
• Web Site: https://search.jamas.or.jp/link/ui/1988000847
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可