Color cathodoluminescence images and cathodoluminescence spectra analysis of biological materials.
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- ITOH TADASHI
- Department of Anatomy, Faculty of Medicine, Kyoto University
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- FUJIMOTO TOYOSHI
- Department of Anatomy, Faculty of Medicine, Kyoto University
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- KOIKE HIROTAMI
- Akashi Seisakusho Ltd.
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- INOUE TAKAO
- Department of Anatomy, Tottori University, School of Medicine
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- OGAWA KAZUO
- Department of Anatomy, Faculty of Medicine, Kyoto University
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In an attempt to detect cathodoluminescence (CL), an Akashi scanning electron microscope (SEM) ISI-DS130 was remodeled into a cathodoluminescence electron microscope (CEM or fluorescence electron microscope FEM). A newly shaped mirror, combining three half ellipsoidal mirrors, was located over the specimen holder of the top stage for color CL use. The bottom stage was modified with a half ellipsoidal mirror, photomultiplier tube (PMT) and monochrometer, so that not only CL images, but also CL spectra could be obtained. An effective specimen-preparation method, known as the simple cryo-SEM method (24), was devised for CL observation.<br>With these instruments and methods, color CL images and CL spectra of the biological substances were analyzed. Ito cells of vitamin A (vit A) -loaded rat liver and zona fasciculata cells of rat adrenal cortex emitted intense CL from their lipid droplets (LD). In order to identify the origin of this CL emission, analysis of the color CL images and the CL spectra were available.
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 19 (5), 621-633, 1986
日本組織細胞化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204863447680
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- NII論文ID
- 110003150014
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- NII書誌ID
- AA00508022
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- ISSN
- 13475800
- 00445991
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可