Immunohistochemical detection of human cellular yes gene (c-yes-1) messenger RNA by a non-radioactive synthesized oligodeoxynucleotide probe.
-
- SUGAWARA ISAMU
- Department of Pathology, The Institute of Medical Science, The University of Tokyo
-
- UCHINO KENJI
- Department of Pathology, The Institute of Medical Science, The University of Tokyo
-
- KOJI TAKEHIKO
- Department of Cell Biology, Tokai University School of Medicine
-
- SUKEGAWA JUN
- Department of Cancer Prevention, The Institute of Medical Science, The University of Tokyo
-
- MORI SHIGEO
- Department of Pathology, The Institute of Medical Science, The University of Tokyo
-
- NAKANE PAUL K.
- The Third Department of Anatomy, Nagasaki University School of Medicine
この論文をさがす
抄録
A synthetic 39-mer anti-sense strand oligodeoxynucleotide complementary to the region of human cellular yes gene (c-yes-1) mRNA that codes for a polypeptide of 543 amino acids (Mr: 60, 801) and the corresponding sense strand 39-mer oligodeoxynucleotide were chemically synthesized by β-cyanoethyl phospho-ramidite method. The probes thymine-thymine (T-T) dimerized by UV irradiation were used for in situ hybridization studies. Various cells were fixed with ethanol-acetic acid (3:1) at room temperature for 20min and hybridization was carried out in 50% formamide for 36hr at 37°C. The good signal was obtained by utilizing anti-T-T dimer serum (IgG frafction), peroxidase-labeled goat anti-rabbit IgG antibody, and diaminobenzidine-4-HCl and H2O2 as substrates. The anti-sense strand synthetic probe hybridized with cellular RNA of cultured cells with c-yes-1 mRNA, while the sense strand synthetic probe did not. The c-yes-1 mRNA was clearly detected in the cytoplasm of c-yes-1 gene product-expressing cultured cells by in situ hybridization using a T-T dimerized synthetic probe. Synthetic T-T dimerized oligodeoxynucleotides appear to constitute attractive reagents for in situ hybridization studies when supported by appropriate control procedures.
収録刊行物
-
- Acta Histochemica et Cytochemica
-
Acta Histochemica et Cytochemica 22 (5), 549-559, 1989
日本組織細胞化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679837692288
-
- NII論文ID
- 110003150533
-
- NII書誌ID
- AA00508022
-
- COI
- 1:CAS:528:DyaK3cXhtFemtLY%3D
-
- ISSN
- 13475800
- 00445991
-
- HANDLE
- 10069/23708
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- Crossref
- NDL-Digital
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可