Distribution of Inducible Nitric Oxide Synthase, Interleukin-1.BETA., and Interleukin-1 Receptor in the Temporomandibular Joint of Normal Rats.

• Masuda Keitaro F.

  Department of Removable Prosthetic Dentistry, Graduate School of Dental Science, Kyushu University Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University

• Yamaza Takayoshi

  Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University

• Tsukiyama Yoshihiro

  Department of Removable Prosthetic Dentistry, Graduate School of Dental Science, Kyushu University

• Murakami Rie

  Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University Department of Orthodontics, Graduate School of Dental Science, Kyushu University

• Nishijima Katsushi

  Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University

• Kido Mizuho A.

  Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University

• Koyano Kiyoshi

  Department of Removable Prosthetic Dentistry, Graduate School of Dental Science, Kyushu University

• Tanaka Teruo

  Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University

抄録

Nitric oxide (NO) is generated from <sc>l</sc>-arginine by NO synthase (NOS) and has multiple functions under both physiological and pathological conditions. One isoform of NOS, inducible NOS (iNOS) is expressed in the cells such as macrophages after induction with various cytokines or mechanical stress. In this study, we investigated the distribution of iNOS, interleukin-1β (IL-1β) and its receptor, the IL-1 receptor (IL-1R), in the synovial membrane of the temporomandibular joint (TMJ) of normal rats using immunolight and immunoelectron microscopy. By light microscopy, an immunopositive reaction for iNOS and IL-1β was found in the superficial cells of the synovial membrane of both the anterior and posterior portions of the articular disc. Immunoelectron microscopy revealed that iNOS-immunoreactive products were deposited in the cytoplasm and vesicles, and on the plasma membrane of type-A (macrophage-like) and B (fibroblast-like) cells of the superficial layer. IL-1R-positive products were found both on the plasma membrane and in the vesicles of type-A cells of the synovial lining, and were observed in macrophages in the sublining layer. These results reveal that iNOS and IL-1β localize to the synovial membrane of the rat TMJ under physiological conditions. Therefore, it is likely that autocrine/paracrine effects of IL-1 β induce NO generation by iNOS via the IL-1R in type-A cells. It is considered that cytokine-induced NO may play an important role in the physiological maintenance, e.g. self-protection, by synovial lining cells of the synovial membrane in the TMJ.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 35 (1), 11-21, 2002

  日本組織細胞化学会

キーワード

• iNOS
• IL-1β
• IL-1 receptor
• Synovial lining cells
• TMJ

詳細情報

• CRID: 1390001204863279616
• NII論文ID: 110003150867
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.35.11
• COI: 1:CAS:528:DC%2BD38XktlOlsLY%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/35/1/35_1_11/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10803894
• Web Site: https://search.jamas.or.jp/link/ui/2002236899
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可