Localization of the Endogenous Cysteine Proteinase Inhibitor, Cystatin C, and the Cysteine Proteinase, Cathepsin B, to the Junctional Epithelium in Rat Gingiva

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Author(s)

    • YAMAZA Takayoshi
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • MINO Satoya
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • ATSUTA Ikiru
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • DANJO Atsushi
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • KAGIYA Tadayoshi
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • NISHIJIMA Katsushi
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • ZANG Jin-Qi
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • KIDO Mizuho A.
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science
    • TANAKA Teruo
    • Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science

Abstract

The junctional epithelium (JE) is a primary site of defense against periodontal pathogens. Cystatin C is an endogenous inhibitor of cysteine proteinases such as cathepsin B and also has antibacterial actions against periodontal pathogens. However, the distribution and role of cystatin C in JE have not been clarified. To investigate the function of cystatin C in the host defense at dentogingival junction, we examined the immunolocalization of cystatin C and cathepsin B in rat gingiva using light and electron microscopy. The JE (particularly the coronal portion) was immunopositive for cystatin C, and immunoelectron microscopy revealed that cystatin C was localized to the vesicular, granular, and vacuolar compartments of JE cells. The pattern of cathepsin B immunoreactivity in JE cells resembled that of cystatin C. Both cystatin C and cathepsin B appeared to be localized to endosomal/lysosomal compartments within JE cells. These findings suggest that cystatin C regulates cysteine proteinase activity and exerts antibacterial effects both in the lysosomal compartments of JE cells and in the intercellular spaces of the JE. Cystatin C is thus able to participate in host defense against periodontal pathogens at the dentogingival junction.<br>

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 38(2), 121-129, 2005-04-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  49

Codes

  • NII Article ID (NAID)
    110003161238
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  NII-ELS  J-STAGE  NDL-Digital 
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