新しく開発された超還元性水の各種細菌に対する殺菌効果と細胞毒性について

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  • Bactericidal Effect against Several Genera of Bacteria and Cytotoxicity of Newly Developed Super Reductive Water.

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The purpose of this study was to investigate the bactericidal effect against several genera of bacteria and fungi of the newly developed super reductive water (SRW) and its cytotoxicity. SRW has -310mV oxidation reduction potential when the pH is 12.0. We used 14 strains of 11 genera of bacteria and 1 strain of fungi. Each genus of bacteria (initial concentration of 102 cells/ml) was incubated with SRW for one and ten min. at room temperature. Then the survival rate was determined by colony formation. For the genus E. coli, P. aeruginosa, A. actinomycetemcomitans, F. nucleatum, and P. gingivalis, the number of cells that survived less than 10102 cells/ml, but for the genus S. aureus, S. epidermidis, S. mutans, S. salivarius, S. sanguis, E. faecalis, L. acidophilus, B. subutilis and C. albicans, cell survival was more than 102 cells/ml. The bactericidal activity against E. coli was not reduced in the presence of serum or thiourea. The cytotoxicity of SRW was tested by alamar blue assay with L 929 cell lines established from subcutaneous tissue in C 3 H mice. The cytotoxicity (LD 50) of SRW determined by alamar blue assay was 33w/w%. These results suggested that newly developed SRW has an excellent bactericidal effect against only gram-negative bacteria and lower cytotoxicity, and that the application of SRW is restricted to the disinfection of gramnegative bacteria.

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