強酸性電解水の細胞毒性について

DOI 被引用文献16件 参考文献22件

書誌事項

タイトル別名
  • Cytotoxicity of aqua-oxidized water.

この論文をさがす

抄録

Aqua-oxidized water (AOW) which is produced by electro-disintegration of 10-20% NaCl solution, is a bactericidal agent. The bactericidal activity is attributed to Cl2, HOCl, superoxide anion, and acidification (pH2.3), but have not been clarified. Herein, the cytotoxicity of AOW was tested by flow cytometric assay, colony formation, and MTT assay with L929 cell lines stemmed from subcutaneous tissue in C3H mice. The flow cytometric assay was performed using the fluorescein diacetate (FDA)-propidium iodide (PI) double staining technique followed by the trypan blue exclusion test. After FDA-PI staining, the cells were analyzed by FACStar plus and LYSIS II software. The colony formation test and MTT assay were performed using the usual methods. Cytotoxicity (LD50) determined by (1) Flow cytometry was 25W/W%(non-serum), and 43W/W%(10% serum), that by (2) colony formation was 25W/W%, and that by (3) MTT assay was 20W/W%, The cytotoxic effect of AOW remained active even in the presence of a high percentage of 5-20% serum. These findings suggest that AOW is cytotoxic to fibroblasts and the application of AOW to humans should be confined to the external area of the body.

収録刊行物

被引用文献 (16)*注記

もっと見る

参考文献 (22)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ