スーパーオキシドアニオンラジカルにより牛血清アルブミン上にチイルラジカルが発生する

• 宮本 有香

  夙川学院短期大学

• 西村 公雄

  同志社女子大学生活科学部

書誌事項

タイトル別名

• Generation of the Thiyl Radical in Bovine Serum Albumin by the Superoxide Anion Radical
• スーパーオキシドアニオンラジカル ニ ヨリ ギュウ ケッセイ アルブミン ジョウ ニ チイルラジカル ガ ハッセイ スル

抄録

The mechanism for the beneficial effects of vitamin C (ascorbic acid, AsA) on the quality of the heatinduced fish gel, kamaboko, has been proposed by Nishimura et al. to involve the production of the thiyl radical (S·). However, the detection of S· in myosin is very difficult, because its large molecular weight (about 500, 000) produces a strained electron spin resonance (ESR) signal. The generation of S· in myosin has therefore not yet been elucidated. We conducted an ESR analysis with bovine serum albumin (BSA) which is a lower-molecular-weight protein (about 60, 000). Although the signal with four main peaks that was observed under illumination at 650, 000 lx in a mixture containing riboflavin, a spintrapping reagent, and reduced BSA by dithiothreitol, non-reduced BSA produced only a small signal. The intensity of the signal obtained by using reduced BSA, however, decreased in response to superoxide dismutase (SOD; 200 units/ml), while inactivated SOD had little effect. These results suggest that S· could be produced in protein by the action of O₂^-.

収録刊行物

• 日本家政学会誌

  日本家政学会誌 56 (7), 457-462, 2005

  一般社団法人 日本家政学会

キーワード

• ビタミンC
• タンパク質
• 牛血清アルブミン
• スーパーオキシドアニオンラジカル
• チイルラジカル
• 電子スピン共鳴

詳細情報

• CRID: 1390282681310009600
• NII論文ID: 110003167109
• NII書誌ID: AN10040097
• DOI: 10.11428/jhej1987.56.457
• ISSN: 18820352
• ISSN: 09135227
• NDL書誌ID: 7378084
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7378084
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10583649
• Web Site: https://search.jamas.or.jp/link/ui/2005253757
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • NDL-Digital
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可