Rapid Production of a Histocompatible Colony of Xenopus laevis by Gynogenetic Procedure(Genetics) :

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Gynogenetic diploid individuals were produced in the frog Xenopus laevis, by means of a brief refrigeration of eggs inseminated by UV-irradiated sperm. Starting with a wild female, this procedure was repeated for 4 generations, and the immunogenetic relationship among siblings of each generation was tested by reciprocal skin grafting and mixed lymphocyte reaction (MLR). All skin grafts exchanged among control, nongynogenetic sibling frogs were rejected within 30 days. In contrast, the survival times of the skin grafts exchanged among siblings of gynogens F_1 and their progeny F_2 were highly variable, either in acute (<30 days) or chronic (30-120 days) terms, including a long-term survival for over 120 days. MLR analyses of gynogenetic F_2 siblings also proved that the loci which control the acute skin graft rejection and the MLR are closely linked with each other, together with a high incidence of crossing-over between these loci and the centromere. A gynogenetic F_2 frog which showed an acute response against intrasibling grafts was selected as a putative homozygote of major histocompatibility complex (MHC), to produce the gynogenetic F_3. All F_3 responded in chronic fashion to intrasibling skin grafts, and exhibited negative MLR in intrasibling tests. In addition, all gynogenetic F_4 frogs derived from a randomly selected F_3 mother tolerated sibling skin grafts for more than 100 days. These results indicate that an immunogenetically defined, fairly pure colony of Xenopus laevis can be obtained in 4 gynogenetic generations, provided that the MHC-homozygote is appropriately selected.

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  • Zoological science

    Zoological science 2 (1), 71-79, 1985-02-15

    Zoological Society of Japan

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