Cloning of the Gene for the Thyrotropin β Subunit in the Japanese Crested Ibis, Nipponia nippon

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Author(s)

    • Aotsuka Tadashi AOTSUKA Tadashi
    • Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University
    • Ishii Susumu
    • Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University

Abstract

We isolated a putative gene for the thyrotropin β subunit (TSHβ) from two types of genomic libraries of the Japanese crested ibis, Nipponia nippon. Exon-intron structure was deduced by comparing the determined sequence with those of TSH β cDNA of other birds. The deduced amino acid sequence shows extensive similarities to those of the other birds, which assures our assumption that the acquired nucleotide sequence represents the TSHβ gene. The assembled genomic fragment is 4192 bp in size and consists of 1937 bp of putative 5 flanking region followed by exon-intron structure with three exons and two introns, similar to those observed in rat, human and goldfish counterparts. Locations of introns are also similar to those in mammals and goldfish. Comparison of the 5 flanking region of the ibis TSHβ gene with those of mammals reveals that several regulatory sequences, such as negative thyroid hormone responsive element (nTRE), Pit-1 responsive element, and AP-1 responsive element, which were characterized in mammalian TSHβ genes, are also found in the promoter region. This is the first report on the exon-intron structure and 5 flanking region of the TSHβ gene in an avian species.

Journal

  • ZOOLOGICAL SCIENCE

    ZOOLOGICAL SCIENCE 20(2), 203-210, 2003-02-01

    The Zoological Society of Japan

References:  19

Codes

  • NII Article ID (NAID)
    110003372937
  • NII NACSIS-CAT ID (NCID)
    AA10545874
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02890003
  • NDL Article ID
    6616827
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z53-Y164
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE  NDL-Digital 
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