Synthetic Decarboxylated S-Adenosyl-L-methionine as a Substrate for Aminopropyl Transferases
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- Dejima Hideki
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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- Kobayashi Masaki
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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- Takasaki Hideki
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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- Takeda Noboru
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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- Shirahata Akira
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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- Samejima Keijiro
- Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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Synthetic decarboxylated S-adenosyl-L-methionine (dcAdoMet), a mixture of the absolute configuration of S and R at the sulfonium center, was evaluated as a substrate for the measurement of spermidine synthase activity. The diastereomers were separated by HPLC with an isocratic elution, and the constant for racemization at the sulfur was determined to be 2.4×10−6 s−1 at 37 °C and pH 1.5 for the first-eluted biologically active isomer (S-dcAdoMet) and 2.0×10−6 s−1 for the second-eluted biologically inactive isomer (R-dcAdoMet). The peak area ratio of S-dcAdoMet to R-dcAdoMet of 48 to 52 in HPLC supported the different racemization constants. Similar substrate activity of dcAdoMet to that of S-dcAdoMet was demonstrated by enzymatic spermidine synthesis. It was shown from the result that the racemized [methyl-14C]dcAdoMet prepared in this report was useful for measuring spermidine synthase activity.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 26 (7), 1005-1008, 2003
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679601583104
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- NII論文ID
- 110003608563
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3sXmsFWntrw%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6583598
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- PubMed
- 12843627
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可