Antioxidant Activities of Chitobiose and Chitotriose

• Chen An-Shu

  Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University

• Taguchi Tadao

  Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University

• Sakai Kazuo

  Yaizu Suisankagaku Industry Co.

• Kikuchi Kazuaki

  Yaizu Suisankagaku Industry Co.

• Wang Min-Wei

  Department of Pharmacology, School of Pharmacy, Shenyang Pharmaceutical University

• Miwa Ichitomo

  Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University

抄録

Chitooligosaccharides, the oligomers made up of β-1,4-linked D-glucosamine, are obtained by partial hydrolysis of chitosan, a deacetylation product of chitin. The antioxidant activity of various chitooligosaccharides was tested in vitro with aminoguanidine, pyridoxamine, and Trolox as reference compounds. Hydroxylation of benzoate to salicylate by H₂O₂ in the presence of Cu²⁺ was effectively inhibited by chitobiose, chitotriose, aminoguanidine, pyridoxamine, and Trolox (their IC₅₀ values=18, 80, 85, 10, and 95 μM, respectively), whereas glucosamine and N-acetylchito-oligosaccharides (di-N-acetylchitobiose and tri-N-acetylchitotriose) did not show any inhibitory activity. Chitobiose and chitotriose were more potent than the 3 reference compounds in scavenging hydroxyl radicals produced by photolysis of zinc oxide: IC₅₀ values of the 2 oligomers were 30 and 55 μM, respectively. Such a scavenging activity of these 2 chitooligomers was also shown by the use of another system, a mixture of Fe³⁺/EDTA/ascorbate/H₂O₂, for producing hydroxyl radicals. Only chitobiose and Trolox, of the 10 compounds tested, had the ability to scavenge superoxide radicals generated by a non-enzymatic system using phenazine methosulfate and NADH. Taken together with our unpublished observation that chitobiose and chitotriose are appreciably absorbed from the intestine of rats, the present results suggest that these 2 chitooligosaccharides would act as effective antioxidants in vivo when orally ingested.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 26 (9), 1326-1330, 2003

  公益社団法人 日本薬学会

キーワード

• chitobiose
• chitotriose
• antioxidant activity
• chitooligosaccharide
• aminoguanidine
• pyridoxamine

詳細情報

• CRID: 1390001204628495744
• NII論文ID: 110003608596
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.26.1326
• COI: 1:STN:280:DC%2BD3svislyjtw%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 6658268
• PubMed: 12951480
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6658268
• Web Site: http://www.jstage.jst.go.jp/article/bpb/26/9/26_9_1326/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004021269
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可