Regulation of Ribozyme Activity with Short Oligonucleotides

• Komatsu Yasuo

  National Institute of Advanced Industrial Science and Technology, Japan

Abstract

Both hammerhead and hairpin ribozymes, which are well known as small catalytic RNAs, can catalyze either cleavage or ligation of RNA with sequence specificity. Although they have been applied to gene therapy and gene discovery, by cutting the mRNAs of target genes, these ribozymes normally do not have the ability to regulate their catalytic activities. Therefore, allosteric control has been applied to the ribozymes. Watson–Crick base pairing has superior ability in molecular recognition, and the sequence of nucleic acids makes diversity of molecules possible. Therefore, we have tried to construct a new ribozyme of which activity is regulated by a specific oligonucleotide. The allosteric ribozyme has potentials of not only regulation of activity but also a sensor for gene expression.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 27 (4), 457-462, 2004

  The Pharmaceutical Society of Japan

Keywords

• ribozyme
• regulation
• oligonucleotide
• RNA

Details

• CRID: 1390282679603079424
• NII Article ID: 110003608786
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.27.457
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 6896721
• PubMed: 15056847
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6896721
• Web Site: http://www.jstage.jst.go.jp/article/bpb/27/4/27_4_457/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed