Quercetin Suppresses Bone Resorption by Inhibiting the Differentiation and Activation of Osteoclasts

• Woo Je-Tae

  Department of Biological Chemistry, Chubu University

• Nakagawa Hiroshi

  Department of Biological Sciences, Tokyo Institute of Technology

• Notoya Michitaka

  Department of Biological Sciences, Tokyo Institute of Technology

• Yonezawa Takayuki

  Department of Biological Chemistry, Chubu University

• Udagawa Nobuyuki

  Department of Biochemistry, School of Dentistry, Matsumoto Dental University

• Lee In-Seon

  In-Seon, Lee Center for Traditional Microorganism Resources, Keimyung University

• Ohnishi Motoko

  Department of Biological Chemistry, Chubu University

• Hagiwara Hiromi

  Department of Biological Sciences, Tokyo Institute of Technology

• Nagai Kazuo

  Department of Biological Chemistry, Chubu University

抄録

Although quercetin has suppressed bone resorption in several animal studies, its target cells and the mechanism of its action related to bone resorption has not been fully elucidated. We investigated the effect of quercetin on the differentiation and activation of osteoclasts. We used cocultures of mouse spleen cells and ST2 cells, and cultures of osteoclast progenitor cells {M-CSF-dependent (MD) cells from mouse bone marrow and murine monocytic RAW 264 (RAW) cells}. Quercetin dose-dependently inhibited osteoclast-like (OCL) cell formation at 2—5 μM concentration in both the coculture and MD cell culture. Quercetin inhibited the increase of tartrate-resistant acid phosphatase (TRAP) activity of mononuclear preosteoclasts (pOCs) induced by receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) in both MD and RAW cell cultures. Quercetin reversely induced the disruption of actin rings in OCLs. Quercetin also suppressed both pit formation induced by osteoclasts on dentine slices and PTH-stimulated ⁴⁵Ca release in mouse long bone cultures. These results suggest that osteoclast progenitors as well as mature osteoclasts, are quercetin's target cells in relation to bone resorption, and that quercetin's suppressive effect on bone resorption results from both its inhibitory effect on the differentiation of osteoclast progenitor cells into pOCs and from its disruptive effect on actin rings in mature osteoclasts.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 27 (4), 504-509, 2004

  公益社団法人 日本薬学会

キーワード

• quercetin
• osteoclast
• bone resorption
• osteoclast activation
• osteoclast differentiation

詳細情報

• CRID: 1390001204625815552
• NII論文ID: 110003608794
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.27.504
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 6896763
• PubMed: 15056855
• Web Site: http://id.ndl.go.jp/bib/6896763
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6896763
• Web Site: https://www.jstage.jst.go.jp/article/bpb/27/4/27_4_504/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005215714
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可