Effects of Iridoid Total Glycoside from Cornus officinalis on Prevention of Glomerular Overexpression of Transforming Growth Factor Beta 1 and Matrixes in an Experimental Diabetes Model

• Xu Hui-Qin

  Department of Pharmacology, College of Pharmacy, Nanjing University of Traditional Chinese Medicine

• Hao Hai-Ping

  Jiangsu Key Laboratory of Metabolism & Pharmacokinetics, China Pharmaceutical University

抄録

The present study was conducted to determine whether iridoid total glycoside from Cornus officinalis was effective in regulating expression of transforming growth factor beta 1 (TGF-β1) and preventing overdeposition of extracellular matrix (ECM) in a diabetes state. An experimental rat model of diabetic nephropathy (DN) was successfully induced by one intraperitoneal injection of streptozotocin at a dose of 60 mg·kg⁻¹ and maintained for 12 weeks. All rats had free access to standard chow and water. Four groups: normal control, diabetic control, diabetic rats with aminoguanidine treatment and diabetic rats with iridoid total glycoside treatment were used in this experiment. All treatments were administered by intragastric gavage (ig). At the end of the experiment, serum was collected for ELISA determination of TGF-β1 protein level; renal cortex was dissected for reverse transcription polymerase chain reaction (RT-PCR) analysis of its mRNA expression; and immunohistochemistry was introduced to observe ECM deposition. A significantly higher level of protein and mRNA expression of TGF-β1, and also overdeposition of fibronectin and laminin was found in diabetic rats. Both iridoid total glycoside and aminoguanidine were effective in decreasing serum protein level and glomerular mRNA expression of TGF-β1, and in preventing renal overdeposition of fibronectin and laminin. This study suggests that iridoid total glycoside is a beneficial agent for prevention and therapy of DN.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 27 (7), 1014-1018, 2004

  公益社団法人 日本薬学会

キーワード

• <i>Cornus officinalis</i>
• diabetic nephropathy
• transforming growth factor beta 1
• fibronectin
• laminin

詳細情報

• CRID: 1390001204627122688
• NII論文ID: 110003608905
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.27.1014
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/03660567
• NDL書誌ID: 6986620
• PubMed: 15256732
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6986620
• Web Site: http://www.jstage.jst.go.jp/article/bpb/27/7/27_7_1014/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005231963
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可