Analytical Chemical Studies on High-Performance Recognition and Detection of Bio-molecules in Life

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  • IMAI Kazuhiro
    <i>Center for Research and Development, Kyoritsu College of Pharmacy</i>

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Other Title
  • 生命機能の分子認識と捕捉の高精密化に関する分析化学的研究
  • セイメイ キノウ ノ ブンシ ニンシキ ト ホソク ノ コウセイミツカ ニ カンスル ブンセキ カガクテキ ケンキュウ

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Abstract

  In order to understand the mechanism for maintaining life of animals based on the search of dynamics of bio-molecules, I have developed several sensitive and selective methods for their quantification. Using the methods of derivatization with the developed benzofurazan fluorogenic reagents (4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole 4-sulfonate (SBD-F) and etc.) followed by high-performance liquid chromatography (HPLC)—fluorescence detection, a certain kind of biological and clinical importances was demonstrated of chiral bio-molecules (D-amino acids, D-lactic acid and so on), peptides and proteins. The proposed method (derivatization with SBD-F, isolation of the fluorescent proteins by two-dimensional HPLC, enzymatic digestion and identification of the altered proteins by HPLC-mass spectrometry (MS)/MS with database-searching algorithm) for proteomics studies revieled the changed proteins in the islets of Langerhans of the dexamethazone-induced diabetic rats. An importance of catecholamine metabolism on the blood pressure regulation was also suggested by the method of HPLC-chemiluminescence detection of catecholamines and their 3-O-methylmetabolites. A new field of Analytical Chemistry, i.e., Bio-Analytical Chemistry, was also proposed.<br>

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 123 (11), 901-917, 2003

    The Pharmaceutical Society of Japan

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