Relationship between Effects of Phenolic Compounds on the Generation of Free Radicals from Lactoperoxidase-Catalyzed Oxidation of NAD(P)H or GSH and their DPPH Scavenging Ability.

DOI Web Site Web Site PubMed 被引用文献1件 参考文献46件

この論文をさがす

抄録

The influence of various phenolic compounds on the lactoperoxidase (LPO)/hydrogen peroxide (H2O2)-catalyzed oxidation of biochemical reductants such as reduced β-nicotinamide adenine dinucleotide (NADH), reduced β-nicotinamide adenine dinucleotide phosphate (NADPH) or reduced glutathione (GSH) was investigated by electron spin resonance (ESR) spectroscopy. Micromolar quantities of phenolic compounds such as 17β-estradiol, phenol, and p-chlorophenol enhanced the LPO/H2O2-catalyzed oxidation of NAD(P)H or GSH to generate a large amount of superoxide radical (O2·-) or glutathione thiyl radical (GS·), while, phenolic compounds such as quercetin and Trolox C greatly suppressed the generation of O2·- and GS·. In order to elucidate the effects of phenolic compounds on the generation of O2·- and GS·, their quenching activities for a stable radical, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), were investigated by ESR spectroscopy. 17β-Estradiol, phenol, and p-chlorophenol showed very weak scavenging activities for DPPH, but quercetin and Trolox C showed strong activities. This suggests that the ability of phenolic compounds to enhance LPO/H2O2-catalyzed oxidation of NAD(P)H or GSH relates inversely to their ability to quench DPPH. That is, phenolic compounds having weak quenching activity against DPPH may enhance the LPO/H2O2-catalyzed oxidation of NAD(P)H or GSH to generate a large amount of O2·- or GS·.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (46)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ