Cross-Linking of Collagen by Singlet Oxygen Generated with UV-A.

• RYU Akemi

• NARU Eiji

• ARAKANE Kumi

• MASUNAGA Takuji

• SHINMOTO Koichi

• NAGANO Tetsuo

• HIROBE Masaaki

• MASHIKO Shinrou

Bibliographic Information

Other Title

• Cross-Linking of Collagen by Singlet Ox

Abstract

Singlet oxygen (¹O₂), a highly reactive and toxic intermediate, may play a role in photo-induced aging. We examined singlet oxygen generation from hematoporphyrin (HP) with UV-A, by monitoring the emission at 1268nm corresponding to ¹O₂→³O₂. Singlet oxygen was formed HP-dose-dependently in this system. We then investigated the reaction of singlet oxygen generated by UV-A irradiation with collagen, which is related to skin elasticity and softness. Collagen from skin was rapidly and dose-dependently cross-linked by singlet oxygen. The reaction was inhibited by NaN₃, a selective quencher of singlet oxygen. In contrast, SOD (superoxide dismutase) and mannitol had no effect. These results suggested that cross-linking of collagen was caused by UV-A-generated singlet oxygen, not by any other reactive oxygen species. Compared with another multisubunit protein, alcohol dehydrogenase, collagen was cross-linked much more efficiently. Further, the finding that semicarbazide inhibited cross-linking of collagen showed that cross-links were formed between photooxidized histidyl residues and amino groups. Singlet oxygen generated by UV-A irradiation may contribute to cross-linkin of collagen in the process of skin photoagaing.

Journal

• Chemical and Pharmaceutical Bulletin

  Chemical and Pharmaceutical Bulletin 45 (8), 1243-1247, 1997

  The Pharmaceutical Society of Japan

Keywords

• singlet oxygen
• UV-A
• photoagaing
• collagen
• cross-link

Details

• CRID: 1390001204164283776
• NII Article ID: 110003616652
• NII Book ID: AA00602100
• DOI: 10.1248/cpb.45.1243
• COI: 1:CAS:528:DyaK2sXlvFSjtL8%3D
• ISSN: 13475223
• ISSN: 00092363
• ISSN: http://id.crossref.org/issn/00092363
• NDL BIB ID: 4287796
• PubMed: 9301026
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4287796
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/45/8/45_8_1243/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed